Evaluation of Laminar Flow Microbiological Safety Cabinets

Evaluation of laminar flow microbiological safety cabinets.

The microbiological control efficiency of two class 100 laminar down-flow hoods was determined by using aerosols of Bacillus subtilis var. niger spores. The first unit challenged utilized a slanted eyelid to partially enclose the front work opening. This hood showed nearly perfect control of ambient organisms in the work area. It also gave a 10(6) or greater drop in the number of organisms pass...

Safety of portable inoculation cabinets.

Laboratory work with pathogenic microorganisms should be performed in a ventilated safety cabinet. A minimum airflow of 0-5 metres per second through the working aperture has been recommended, with discharge of the exhaust air through a filter, then into the open air (Department of Health and Social Security, 1970). Such a unit must be permanently installed. There are possible advantages to a p...

Policy on Choice of Fumigant for Fumigation of Microbiological Safety Cabinets and Containment Level 3 Suites

Modified laminar flow biological safety cabinet.

Tests are reported on a modified laminar flow biological safety cabinet in which the return air plenum that conducts air from the work area to the high efficiency particulate air filters is under negative pressure. Freon gas released inside the cabinet could not be detected outside by a freon gas detection method capable of detecting 10(-6) cc/s. When T3 bacteriophage was aerosolized 5 cm outsi...

Fluid dynamics of cytotoxic safety cabinets.

This study investigated the specific fluid dynamics characteristics of cytotoxic safety cabinets (CSC), particularly those used in cancer drug reconstitution operations. Measurements taken on site were used to derive characteristic data for these cabinets. An in-depth laboratory investigation of airflows inside another CSC was also conducted. Anemometric values recorded on these two installatio...